Quite often, when providing statistical help for Stat-Ease software users, our consulting team sees an over-selection of effects from two-level factorial experiments. Generally, the line gets crossed when picking three-factor interactions (3FI), as I documented in the lead article for the June 2007 *Stat-Teaser*. In this case, the experimenter picked all the estimable effects when only one main effect (factor B) really stood out on the Pareto plot. Check it out!

In my experience, the true 3FIs emerge only when one of the variables is categorical with a very strong contrast. For example, early in my career as an R&D chemical engineer with General Mills, I developed a continuous process for hydrogenating a vegetable oil. By cranking up the pressure and temperature and using an expensive, noble-metal catalyst (palladium on a fixed bed of carbon), this new approach increased the throughput tremendously over the old batch process, which deployed powered nickel to facilitate the reaction. When setting up my factorial experiment, our engineering team knew better than to make the type of reactor one of the inputs, because being so different, this would generate many complications of time-temperature interactions differing from on process to the other. In cases like this, you are far better off doing separate optimizations and then seeing which process wins out in the end. (Unfortunately for me, I lost this battle due to the color bodies in the oil poisoning my costly catalyst.)

A response must really behave radically to require a 3FI for modeling as illustrated hypothetically in Figures 1 versus 2 for two factors—catalyst level (B) and temperature (D)—as a function of a third variable (E)—the atmosphere in the reactor.

**Figures 1 & 2: 3FI (BDE) surface with atmosphere of nitrogen vs air (Factor E at low & high levels)**

These surfaces ‘flip-flop’ completely like a bird in flight. Although factor E being categorical does lead to a strong possibility of complex behavior from this experiment, the dramatic shift caused by it changing from one level to the other would be highly unusual by my reckoning.

It turns out that there is a middle ground with factorial models that obviates the need for third-order terms: Multiple two-factor interactions (2FIs) that share common factors. The actual predictive model, derived from a case study we present in our Modern DOE for Process Optimization workshop, is:

Yield = 63.38 + 9.88*B + 5.25*D − 3.00*E + 6.75*BD − 5.38*DE

Notice that this equation features two 2FIs, BD and DE, that share a common factor (D). This causes the dynamic behavior shown in Figures 3 and 4 without the need for 3FI terms.

**Figure 3 & 4: 2FI surface (BD) for atmosphere of nitrogen vs air (Factor E at low & high levels)**

This simpler model sufficed to see that it would be best to blanket the batch reactor with nitrogen, that is, do not leave the hatch open to the air—a happy ending.

**Conclusion**

If it seems from graphical or other methods of effect selection that 3FI(s) should be included in your factorial model, be on guard for:

- Over-selection of effects (my first case)
- The need for a transformation (such as log): Be sure to check the Box-Cox plot (always!).
- Outlier(s) in your response (look over the diagnostic plots, especially the residual versus run).
- A combination of these and other issues—ask stathelp@statease.com for guidance if you use Stat-Ease software (send in the file, please).

I never say “never”, so if you really do find a 3FI, get back to me directly.

-Mark (mark@statease.com)

One of the greatest tools developed by Cuthbert Daniel (1976), was the use of the half-normal plot to visually select effects for two-level factorials. Since these designs generally contain no replicates, there is no pure error to use as the base for statistical F-tests. The half-normal plot allows us to visually distinguish between the effects that are small (and normally distributed) versus large (and likely to be statistically significant). The subsequent ANOVA is built on this decision to split the effects into the few that are likely “signal” versus the majority that are likely “noise”.

Often the split between the groups is obvious, with a clear gap between them (see Figure 1), but sometimes it is more ambiguous and harder to decide where to “draw the line” (see Figure 2).

Stat-Ease consultants recommend staying conservative when deciding which effects to designate as the “signal”, and to be cautious about over-selecting effects. In Figure 2, the A effect is clearly different from the other effects and should definitely be selected. The C effect is also separated from the other effects by a “gap” and is probably different, so it should also be included in the potential model terms. The next grouping consists of four three-factor interactions (3FI’s.) Extreme caution should be exercised here – 3FI terms are very rare in most production and research settings. Also, they fall “on the line”, which indicates that they are most likely within the normal probability curve that contains the insignificant effects. These terms should be pooled together to estimate the error of the system. The conservative approach says that choosing A and C for the model is best. Adding any other terms is most likely just chasing noise.

Hints for choosing effects:

- Split the effects into two groups, distinguishing between the “big” and “small” ones, right versus left, respectively
- Start from the right side of the graph – that is where the biggest effects are
- Look for gaps that separate big effects from the rest of the group
- STOP if you select a 3FI term – these are very unlikely to be real effects (throw them back into the error pool)
- Don’t skip a term – if a smaller effect looks like it could be significant, then all larger effects also must be included
- Effects need to “jump off” the line – otherwise they are just part of the normal distribution

Sometimes you have to simply accept that the changes in the factor levels did not trigger a change in the response that was larger than the normal process variation (Figure 3). Note in Figure 3 that the far right points are straddling the straight line. These terms have virtually the same size effect – don’t select the lower one just because it is below the line.

When you are lucky enough to have replicates, the pure error is then used to help position green triangles on the half-normal plot. The triangles span the amount of error in the system. If they go out farther than the biggest effect, that is a clear indication that there are no effects that are larger than the normal process variation. No effects are significant in this case (see Figure 4).

**Conclusion**

The half-normal plot of effects gives us a visual tool to split our effects into two groups. However, the use of the tool is a bit of an art, rather than an exact science. Combine this visual tool with both the ANOVA p-values and, most importantly, your own subject matter knowledge, to determine which effects you want to put into the final prediction model.

Version 13 of Design-Expert® software (DX13) provides a substantial step up on ease of use and statistical power for design of experiments (DOE). As detailed below, it lays out an array of valuable upgrades for experimenters and industrial statisticians. See DX13’s amazing features for yourself via our free, fully functional, trial download at www.statease.com/trial/.

Quite often an experiment leads to promising results that lie just beyond its boundaries. DX13 paves the way via its new wizard for modifying your design space. Press the Augment Design button, select “Modify design space” and off you go. Run through the “Modify Design Space – Reactive Extrusion” tutorial, available via program Help, to see how wonderfully this new wizard works. As diagrammed on its initial screen, the modify-design-space tool facilitates shrinking and moving your space, not just expanding it. And it works on mixture as well as process space.

For assessing measures that come by counts, Poisson regression models fit with greater precision than ordinary methods. Demonstrate this via the “Poisson Regression – Antiseptic” tutorial where Poisson regression proves to be just the right tool for modeling colony forming units (CFU) in a cell culture. This new modeling tool, along with logistic regression for binary responses (introduced in version 12), puts Design-Expert at a very high level for a DOE-dedicated program.

Easily model any response in various ways to readily compare them. Then chose the model most fitting for achieving optimization goals. Simply press the plus **[+]** button on the Analysis branch. The Antiseptic tutorial demonstrates the utility of trying several modeling alternatives, none of which can do better than Poisson regression (but worth a try!).

Optimal (custom) designs work wonderfully well for laying out statistically ideal experiments. However, the numerical levels they produce often extend to an inconvenient number of decimal places. No worries: DX13 provides a new “Round Columns” button—very convenient for central composite and optimal designs. As demonstrated in the Antiseptic tutorial, this works especially well for mixture components—maintaining their proper total while making the recipe far easier for the experimenter to accomplish. Do so either on the basis of significant digits (as shown) or by decimal places.

DX13 makes it far easier to bring in existing data. Simply paste in your data from a spreadsheet (or another statistical program) and identify each column as an input or output. If you paste in headers, right click rows to identify names and units of measure. For example, DX13 enables entry of the well-known Longley data (see the “Historical Data – Unemployment” tutorial for background) directly from an Excel spreadsheet. Easy! Once in Design-Expert, its advanced tools for design evaluation, modeling and graphics can be put to good use.

- The Constraints node now allows you to modify existing limits: Second thoughts? No problem!
- New ribbon with easy access to versatile design-layout features such as Change View, and Hide/Show Columns
- Runs outside the constraints flagged, but still usable for analysis; furthermore, they can be moved back into the valid space via the right-click menu
- Adding verification runs after an analysis no longer invalidates it
- Continuous and discrete numeric factors now indicated in the Design Summary

- Response name now included when copying equations to Excel
- Pearson, Deviance, and Hosmer-Lemeshow goodness-of-it tests added for logistic regression

- New preference for the default layout of the Diagnostics tabs

- Box (and whiskers) Plot for Graph Columns: Another very useful tool for data exploration prior to analysis.
- Control multiple graphs at the same time with the factors tool: Side-by-side interactive views—enlightening!
- Perturbation and trace plots now colored by factor
- New All-Factor graphs option shows only factors selected for the model
- When the number of tick-marks becomes large, only a subset is shown
- For large designs, the Leverage graph scales to maximum value, rather than 1
- When FDS-graph crosshair goes above 80% it changes to black, rather than red

Stat-Ease is here for you during these trying times. We can help you with your design and analysis of experiments, whether at home or in the lab. Please reach out if you have a question, sales@statease.com

**A summary of information that may be important to you**

Access to Design-Expert® software while working at home:

- Download a free trial at www.statease.com/trial/. (Used your DX12 trial already? Ask us!)
- Network users - temporarily bring a license home by checking out a roaming license from your server. Go to www.statease.com/docs/v12/network-installation/#roaming-licenses for more information.
- Need Technical Assistance? Email support@statease.com

Access to FREE educational materials:

- Elearning - learn DOE on demand at the Stat-Ease Academy. Go to www.statease.com/training/academy/ to sign up.
- Hear from our DOE experts: visit our Webinars page at www.statease.com/webinars/ and our YouTube channel, www.youtube.com/channel/UCzf_5--odkQ4w25m5r09bWA
- Dive into Design-Expert software: Tutorials (www.statease.com/docs/v12/tutorials/)

2020 European Conference: Our conference (www.statease.com/events/doe-user-meetings/8th-european-doe-meeting/) is being re-imagined into an online opportunity that will be accessible to our global audience!

To receive information by email, go to www.statease.com/publications/signup/ and signup for our email list.

If you have other needs while transitioning to a new work setup, or an Academic online learning environment, please contact sales@statease.com

**The situation:** You have successfully run an experiment and analyzed the data. The results include a prediction equation with a high predicted R-squared that will be useful for many purposes. How can you share this with colleagues?**The solution:** Design-Expert® software has a little-known but useful “Copy Equation” function that allows you to export the prediction equation to MS Excel so that others can use it for future work, without needing a copy of Design-Expert software. The advantage of using this function is that it brings in all the essential significant digits, including ones not showing on your screen. This accuracy is critical to getting correct predictive values.

- Go to the ANOVA tab for the response. Find the Actual Equation, located in the lower right corner by default.
- Right-click on the equation and select
**Copy Equation**.

3. Open Excel, position your mouse and use Ctrl-V to correctly paste the formula into Excel (Ctrl-V allows the spreadsheet functionality to work.)

4. As shown in the figure (coloration added within Excel), the blue cells allow the user to enter actual factor settings. These values are used in the prediction equation, with the result showing in the yellow cell.

You can also view this process in this video.

Good luck with your experimenting!