Designed Experiments for Life Sciences (2 days)

Section 1—Introduction to Factorial Design

· Brief description of factorial design

· The factorial design planning process

· Basics of factorial design: Microwave popcorn

· Multiple response optimization

Section 2—Enhancements for Full Factorials

· Replicated full factorial (mouse cell assay)

· Explanation of power

· 2⁸ full factorial (assay contaminants)

· Blocking

Section 3—Fractional Factorials

· Fractional factorials

· 2^8-2 fractional factorial (assay contaminants)

· Setup and aliases

· General Factorials

· Immunofluorescent assay optimization

Section 4—Introduction to Response Surface Design

· Factorial with center points (bench reaction)

· Introducing Response Surface Methods “RSM”

· Augmenting to central composite design “CCD”

· “Good” response surface designs

· Sizing for precision

Section 5—Response Surface Methodology

· Instructor-led exercise (fluid bed coating)

· Building the design

· Details of analysis

· Optimization

· "Titration" curve (mouse cell assay revisited)

· Response transformation

Section 6—Response Surface Case Studies

· Drug stability (expiry dating)

· Constrained design space (stent spray coating)

· D-optimal design

Section 7—Introduction to Mixture Design

· What makes a mixture?

· Mixture (Scheffé) polynomials

· Simplex lattice designs (synthetic medium)

· Constrained mixture (antiseptic)

Section 8—Combined Mixture-Process Designs

· Mixture amount (time release coating)

· Combined design (powder coating)

Summary

· Next steps

· Optional exercise

See the public workshop schedule and the DELS course description for further information.