Designed Experiments for Assay Optimization (2 days)

Day 1	Section 1	—	Introduction to Factorial Design
			Brief description of factorial design The factorial design planning process Basics of factorial design (microwave popcorn) Multiple response optimization
Lunch	Section 2	—	Enhancements for Full Factorials
			Replicated full factorial (mouse cell assay) Explanation of power 28 full factorial (assay contaminants) Blocking
	Section 3	—	Fractional Factorials
			Fractional factorials 28-2 fractional factorial (assay contaminants) Setup and aliases Minimum-run resolution V (MR5) designs
			General Factorials
			Immunofluorescent assay optimization
Day 2	Section 4	—	Introduction to Response Surface Design
			Factorial with center points (bench assay) Introducing Response Surface Methods “RSM” Augmenting to central composite design “CCD” “Good” response surface designs Sizing for precision
	Section 5	—	Response Surface Methodology
			Instructor-led exercise (microwave digestion) Building the design Details of analysis Optimization
Lunch	Section 6	—	Advanced Techniques
			User-Defined Response Surface "Titration" curve (mouse cell assay revisited) Split Plot PCR optimization
	Section 7	—	Introduction to Mixture Design
			What makes a mixture? Mixture (Scheffé) polynomials Simplex lattice designs (low cost media) Constrained mixture (moisture determination)
	Section 8	—	Combined Mixture-Process Design
			Reverse phase HPLC Summary Next steps Optional exercise: optimize your assay

See the DEAO course description for further information. To view our public workshops, click here.

Contact Elicia by e-mail or by calling 1.612.746.2038 with any questions.